Consequently, the introduction of the sample solution into the microchip 1a in which micro channel structures are formed can be carried out in a shorter period of time.
因此,形成微型通道结构的微芯片1a内样品溶液的导入可在较短时间内进行。
The ferrous ion test strips will also indicate the concentration of ferrous ions in a sample solution in the same way as the chloride strips.
亚铁离子测试条还可指示样品溶液中的亚铁离子浓度,指示方法与氯离子相同。
The sample solution completely fulfills the four desiderata I listed at the beginning of this article
To install the sample solution, follow the next three steps as outlined in the following set of procedures.
要安装该示例解决方案,请按照下面过程中简要列出的三个步骤进行操作。
The chloride test strips will indicate the concentration of chloride ions in the sample solution and if the area of sample collection and the volume of water is known the concentration can be measured in parts per million or micrograms per millilitre.
氯测试条指示样品溶液中的氯离子浓度,如果已知取样区和水体积,可测量百万分之一或每毫升微克的浓度。
Based on the above, it was confirmed that the microchip nucleic acid amplification reaction according to the present technology not only enables analysis to be carried out simply just by the introduction of a sample solution and the like, but since non-specific nucleic acid amplification is suppressed, also enables highly accurate analysis.
基于上述结果,可以确定的是,根据本技术的微芯片核酸扩增反应不仅能通过导入样品溶液等而使分析变得简单,而且,由于抑制了非特异性核酸扩增反应,实现了高精度分析。
Consequently, a nucleic acid amplification reaction can be started just by supplying the remaining substances required for the nucleic acid amplification reaction and the sample solution including the target nucleic acid amplification strand to the wells 41 to 45.
因此,可通过在井41至45中提供核酸扩增反应所需的剩余物质和包括目标核酸扩增链的样品溶液而开始进行核酸扩增反应。
By forming the substrate layer 11 from an elastic material, when the needle is withdrawn from the introduction part 2 after the sample solution has been introduced, the penetrated location can be naturally sealed due to the self-sealing ability of the substrate layer 11.
如果衬底层11由弹性材料制成,导入样品溶液之后从导入部分2中抽出针时,由于衬底层11的自动密封能力,穿透位置可自然密封。
When the procedure is run with 10 of the sample solution under the above operating conditions, the resolution between the two peaks of sulbenicillin is not less than 2.0.
使用10微升样品溶液在上述操作条件下进行液相色谱法测试程序后,两个磺苄青霉素钠波峰之间的分辨率不低于2.0。
For example, a reagent R including an enzyme may be contained in well 43, and the other components required for the nucleic acid amplification reaction, such as a primer, may be introduced into the microchip 1a-2 by mixing with the sample solution when the nucleic acid amplification reaction starts.
例如,可将含有酶的试剂R装到井43中,核酸扩增反应所需的其它成分,例如,引物,可在开始核酸扩增反应时通过与样品溶液混合而导入微芯片1a-2内。
Consequently, similar to the microchip 1a, when performing a nucleic acid amplification reaction using the microchip 1b, the nucleic acid amplification reaction can be simply performed by introducing just the remaining substances required for the nucleic acid amplification reaction and the sample solution including the target nucleic acid amplification strand into the wells 41 to 45.
因此,与微芯片1a相似,使用微芯片1b进行核酸扩增反应时,可仅通过在井41至45中导入核酸扩增反应所需的剩余物质和包括目标核酸扩增链的样品溶液而进行核酸扩增反应。
Regarding the composition of the reagent solutions, for example, it is preferred to prepare a reagent solution that includes a primer but does not include an enzyme and a reagent solution that includes an enzyme but does not include a primer, because this means the primer and the enzyme do not mix until the sample solution introduced when the nucleic acid amplification reaction is started reaches the well, which suppresses non-specific amplification of the nucleic acid by primer dimers.
对于试剂溶液的成分,例如,优选地,应配置包括引物但不包括酶的试剂溶液和包括酶但不包括引物的试剂溶液,因为这意味着开始核酸扩增反应时导入的样品溶液到达井内之前,引物和酶不会混合,抑制了引物二聚物对核酸产生的非特异性扩增反应。
The sample solution is titrated against a solution of silver nitrate of known concentration.
样品溶液用已知浓度的硝酸银溶液进行滴定。