Place 2 l of the test sample solution (5.6) and 2 l of the reference solution (3.7) on a TLC plate (4.1).
Nanieść 2 l roztworu próbki analitycznej (5.6) i 2 ul roztworu odniesienia (3.7) na płytkę do chromatografii cienkowarstwowej (4.1).
A2= PS or PE peak area of the test sample solution (7.2)
A2 = powierzchnia piku PS lub PE roztworu próbki badanej (ppkt 7.2.)
PS or PE peak area of the test sample solution (7.2)
powierzchnia piku PS lub PE roztworu próbki badanej (ppkt 7.2)
T2= Tryptamine peak area of the test sample solution (7.2).
T2 = powierzchnia piku tryptaminy w roztworze próbki badanej (ppkt 7.2.)
Note: Test sample solution should be stored at 4 ºC until the HPLC analysis is performed.
Uwaga: Do chwili przeprowadzenia analizy HPLC roztwór próbki badanej należy przechowywać w temp. 4 ºC.
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