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DNA and protein quantification were immediately measured at low temperature (4 C) samples taken from the fridge just before the fluorescent liquid sample was pre tested. 分析少量(3-40ul)珍贵样品
ProteomicsTools (Version: 3.0.5) was used to calculate relative ratios of identified peptides among labeled samples using relative peak intensities of released iTRAQ reporter ions in each of the MS/MS spectra, while relative protein quantification among samples was based on weighted ratios of uniquely identified peptides which belonged to the specific individual protein in which sample REF was used as reference.
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Final ratios of protein quantification were then normalized by the median average protein quantification ratio for unequally mixed different labeled samples.
然后通过不均匀混合不同标签样本的中值平均蛋白量化比例,将蛋白质定量的最终比率标准化。
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