Vertaling van "sample solution is introduced" in Chinees
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样品溶液
Further, when the area into which the sample solution is introduced is hermetically sealed by having a lower pressure than atmospheric pressure, at the point when the tip of the needle reaches the introduction part 2, due to the pressure difference between outside the microchip 1a and the introduction part 2, the sample solution in the syringe is automatically sucked into the introduction part 2.
In the microchip 1a-2 according to the present technology, a part of the components required for a nucleic acid amplification reaction are contained in advance in the wells 41 to 45, so that the components included in the reagent R in the wells can be kept separate from the other components until the sample solution is introduced into the wells.
Further, when the area of the microchip 1a into which the sample solution is introduced is hermetically sealed due to having a lower pressure than atmospheric pressure, it is also preferred that the substrate layers 12 and 13 is formed from a gas-impermeable material in order to prevent the entry of air from outside of the microchip 1a to maintain the internal negative pressure.
By thus configuring so that an enzyme is not included in reagent R1 that includes a primer, and so that a primer is not included in reagent R2 that includes an enzyme, the primer and the enzyme do not mix until the sample solution is introduced into the wells, which suppresses the occurrence of primer dimers.
In the microchip 1a, by making the area into which the sample solution is introduced have a pressure lower than atmospheric pressure, the sample solution is sucked up due to the negative pressure inside the microchip when the sample solution is introduced.
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